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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- ARG54731 - Provider product page
- Provider
- Arigo
- Product name
- anti-Cdc25A phospho (Thr507) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH-conjugated phosphospecific peptide around Thr507 of Human CDC25A protein (NP_001780.2).
- Description
- Protein G purified
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 200 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to 6 months. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: extracts from Hela cells, untreated or treated with calyculin A stained with ARG54731 anti-Cdc25A phospho (Thr507) antibody.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: cells transfected with wild type or mutant T507 A of CDC25A stained with ARG54731 anti-Cdc25A phospho (Thr507) antibody. Data courtesy of Dr. Tiebang Kang of Washington University, St. Louis, MO.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: HeLa cells stained with ARG54731 anti-Cdc25A phospho (Thr507) antibody at 1:25 dilution. Alexa Fluor 488-conjugated secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: human cancer tissue stained with ARG54731 anti-Cdc25A phospho (Thr507) antibody. BC = breast carcinoma; HC = hepatocarcinoma.
