620702

antibody from BioLegend

Targeting: CDC25A

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

620702

Provider

BioLegend

Product name

Purified anti-Cdc25A Phospho (Ser17)

Antibody type

Polyclonal

Antigen

Modified peptide

Description

Cdc25A (also know as M-phase inducer phosphatase 1 and dual specificity phosphatase Cdc25A) is a 65 kD MPI phosphatase containing a rhodanese domain. This nuclear protein functions as a dosage-dependent inducer in mitotic control required for the progression of cell cycle. Cdc25A activates Cdc2 by dephosphorylation, dephosphorylates CDK2 in complex with cyclin E. Cdc25A is timulated by cyclins B, downregulated by Chk1 and Cds1/Chk2 phosphorylation. This phosphatase has been shown to interact with Cdc2, cyclin B, Cdk2, Chk1, and Chk2. The Poly6207 antibody recognizes phosphorylated human Cdc25A (Ser17) and has been shown to be useful for Western blotting.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

200 µl

Storage

Upon receipt, store frozen at -20° C.

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Hela cells were treated with 300 μM mimosine for 16 hrs, then placed in complete media (lane 1) or media containing 200 ng/ml nocodazole (lane 2) for an additional 18hrs. Nuclear extract was prepared, resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit antibodies reactive against phosphorylated Cdc25A (Ser17). Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.

Conjugate

Unconjugated