Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AP1194c - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP1194c, RRID:AB_2239028
- Product name
- SET7 (SET9) Antibody (Center)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- The anti-SET9 Pab (Cat. #AP1194c) is used in Western blot to detect SET9 in mouse brain tissue lysate.
- Primary Ab dilution
- 1:1000
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of SET9(arrow) using rabbit polyclonal SET9 Antibody(Cat. #AP1194c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SET9 gene (Lane 2) (Origene Technologies).
- Primary Ab dilution
- 1:1000
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- SET9 Antibody (S174) (Cat. #AP1194c) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the SET9 antibody detected the SET9 protein (arrow).
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma."
- Primary Ab dilution
- 1:50~100
