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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [7]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-78287 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LHX2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: 293T, A431, HeLa, HepG2, Neuro 2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12, mouse brain, mouse eye
- Concentration
- 0.97 mg/mL
Submitted references Chromosome 10q26-driven age-related macular degeneration is associated with reduced levels of HTRA1 in human retinal pigment epithelium.
Williams BL, Seager NA, Gardiner JD, Pappas CM, Cronin MC, Amat di San Filippo C, Anstadt RA, Liu J, Toso MA, Nichols L, Parnell TJ, Eve JR, Heinz S, Hayes MGB, Bartel PL, Zouache MA, Richards BT, Hageman GS
Proceedings of the National Academy of Sciences of the United States of America 2021 Jul 27;118(30)
Proceedings of the National Academy of Sciences of the United States of America 2021 Jul 27;118(30)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LHX2 in A) Neuro2A whole cell lysate, B) GL261 whole cell lysate, C) C8D30 whole cell lysate, D) NIH-3T3 whole cell lysate, E) BCL-1 whole cell lysate, F) BCL-1 whole cell lysate, G) Raw264.7 whole cell lysate, H) C2C12 whole cell lysate. Samples were separated with 10% SDS-PAGE and incubated with LHX2 polyclonal antibody (Product # PA5-78287) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LHX2 in A) 293T whole cell lysate, B) A431 whole cell lysate, C) HeLa D HepG2 whole cell lysate. Samples were separated with 10% SDS-PAGE and incubated with LHX2 polyclonal antibody (Product # PA5-78287) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LHX2 in various tissue extracts using 50 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with LHX2 polyclonal antibody (Product # PA5-78287) using a dilution of 1:10,000 (left panel) and 1:5000 (right panel) followed by HRP-conjugated anti-rabbit IgG.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- LHX2 Polyclonal Antibody detects LHX2 protein by western blot analysis. A. Neuro2A whole cell lysate/extract. B. GL261 whole cell lysate/extract. C. C8D30 whole cell lysate/extract. D. NIH-3T3 whole cell lysate/extract. E. BCL-1 whole cell lysate/extract. F. BCL-1 whole cell lysate/extract. G. Raw264.7 whole cell lysate/extract. H. C2C12 whole cell lysate/extract.10% SDS-PAGE. LHX2 Polyclonal Antibody (Product # PA5-78287) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using LHX2 Polyclonal Antibody (Product # PA5-78287). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with LHX2 Polyclonal Antibody (Product # PA5-78287) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using LHX2 Polyclonal Antibody (Product # PA5-78287). Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membranes were blotted with LHX2 Polyclonal Antibody (Product # PA5-78287) diluted at 1:10,000 (left panel) and 1:5,000 (right panel). The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- LHX2 Polyclonal Antibody detects LHX2 protein by western blot analysis. A. Neuro2A whole cell lysate/extract. B. GL261 whole cell lysate/extract. C. C8D30 whole cell lysate/extract. D. NIH-3T3 whole cell lysate/extract. E. BCL-1 whole cell lysate/extract. F. BCL-1 whole cell lysate/extract. G. Raw264.7 whole cell lysate/extract. H. C2C12 whole cell lysate/extract.10% SDS-PAGE. LHX2 Polyclonal Antibody (Product # PA5-78287) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen) analysis of LHX2 was performed in frozen-sectioned rat E13.5 brain tissue using LHX2 Polyclonal Antibody (Product # PA5-78287) at a dilution of 1:250 (Green). Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- LHX2 Polyclonal Antibody detects LHX2 protein expression by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: LHX2 protein stained by LHX2 Polyclonal Antibody (Product # PA5-78287) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250. Blue: Fluoroshield with DAPI .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4. Epigenetic analysis of Chr10 AMD-associated locus. ( A and B ) ChIP-Seq analysis of H3K27Ac and H3K4me1 in adult human RPE ( A ) or retina ( B ) tissue from two independent homozygous nonrisk donors (-1 and -2). Normalized fragment coverage is displayed. The gray region represents the 4-kb HTRA1 regulatory region, and the red line marks the location of rs36212733. ( C ) Consensus Lhx2 DNA binding motif and predicted Lhx2 binding site (underlined) within the 4-kb HTRA1 regulatory region on Chr10 overlapping the rs36212733 SNP. ( D ) EMSA analysis for SNP-genotype-specific binding of nuclear extracts from HEK293 cells with (+) or without (-) transient Lhx2 overexpression to oligonucleotide (oligo) probes with the risk or nonrisk SNPs at rs36212732 and rs36212733. Anti-Lhx2 antibody or unlabeled probe was included in some reactions, as indicated. Red asterisk indicates band found in Lhx2-containing nuclear extracts but not extracts without Lhx2 overexpression. Green asterisk indicates the band supershifted with addition of Lhx2 antibody to the reaction. ( E ) Same as in D , except that increasing amounts of biotin-labeled probe were included in the reaction mixture.
