Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PAB4562 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4562, RRID:AB_1578570
- Product name
- PDK2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PDK2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Fenofibrate suppresses growth of the human hepatocellular carcinoma cell via PPARĪ±-independent mechanisms.
Regulation of pyruvate dehydrogenase in the common killifish, Fundulus heteroclitus, during hypoxia exposure.
Estrogen-related receptors stimulate pyruvate dehydrogenase kinase isoform 4 gene expression.
Reperfusion-induced translocation of deltaPKC to cardiac mitochondria prevents pyruvate dehydrogenase reactivation.
Yamasaki D, Kawabe N, Nakamura H, Tachibana K, Ishimoto K, Tanaka T, Aburatani H, Sakai J, Hamakubo T, Kodama T, Doi T
European journal of cell biology 2011 Aug;90(8):657-64
European journal of cell biology 2011 Aug;90(8):657-64
Regulation of pyruvate dehydrogenase in the common killifish, Fundulus heteroclitus, during hypoxia exposure.
Richards JG, Sardella BA, Schulte PM
American journal of physiology. Regulatory, integrative and comparative physiology 2008 Sep;295(3):R979-90
American journal of physiology. Regulatory, integrative and comparative physiology 2008 Sep;295(3):R979-90
Estrogen-related receptors stimulate pyruvate dehydrogenase kinase isoform 4 gene expression.
Zhang Y, Ma K, Sadana P, Chowdhury F, Gaillard S, Wang F, McDonnell DP, Unterman TG, Elam MB, Park EA
The Journal of biological chemistry 2006 Dec 29;281(52):39897-906
The Journal of biological chemistry 2006 Dec 29;281(52):39897-906
Reperfusion-induced translocation of deltaPKC to cardiac mitochondria prevents pyruvate dehydrogenase reactivation.
Churchill EN, Murriel CL, Chen CH, Mochly-Rosen D, Szweda LI
Circulation research 2005 Jul 8;97(1):78-85
Circulation research 2005 Jul 8;97(1):78-85
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PDK2 (arrow) using PDK2 polyclonal antibody (Cat # PAB4562). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDK2 gene (Lane 2) (Origene Technologies).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PDK2 polyclonal antibody (Cat # PAB4562) in mouse intestine tissue lysate (35 ug/lane). PDK2 (arrow) was detected using the purified PDK2 polyclonal antibody (Cat # PAB4562).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with PDK2 polyclonal antibody (Cat # PAB4562) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma .
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
