Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- ABIN954072 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Pyruvate Dehydrogenase Kinase, Isozyme 2 (PDK2) antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant Human PDK2 protein.
- Description
- Protein A column, followed by protein affinity purification
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Genotype-phenotype correlation in children with autosomal dominant polycystic kidney disease.
Mitochondrial mutations contribute to HIF1alpha accumulation via increased reactive oxygen species and up-regulated pyruvate dehydrogenease kinase 2 in head and neck squamous cell carcinoma.
Pivotal role of the C-terminal DW-motif in mediating inhibition of pyruvate dehydrogenase kinase 2 by dichloroacetate.
The rat sperm proteome characterized via IPG strip prefractionation and LC-MS/MS identification.
Fencl F, Janda J, Bláhová K, Hríbal Z, Stekrová J, Puchmajerová A, Seeman T
Pediatric nephrology (Berlin, Germany) 2009 May;24(5):983-9
Pediatric nephrology (Berlin, Germany) 2009 May;24(5):983-9
Mitochondrial mutations contribute to HIF1alpha accumulation via increased reactive oxygen species and up-regulated pyruvate dehydrogenease kinase 2 in head and neck squamous cell carcinoma.
Sun W, Zhou S, Chang SS, McFate T, Verma A, Califano JA
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Jan 15;15(2):476-84
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Jan 15;15(2):476-84
Pivotal role of the C-terminal DW-motif in mediating inhibition of pyruvate dehydrogenase kinase 2 by dichloroacetate.
Li J, Kato M, Chuang DT
The Journal of biological chemistry 2009 Dec 4;284(49):34458-67
The Journal of biological chemistry 2009 Dec 4;284(49):34458-67
The rat sperm proteome characterized via IPG strip prefractionation and LC-MS/MS identification.
Baker MA, Hetherington L, Reeves G, Müller J, Aitken RJ
Proteomics 2008 Jun;8(11):2312-21
Proteomics 2008 Jun;8(11):2312-21
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of PDK2 Antibody Cat.-No AP53243PU-N in Jurkat cell line lysates (35ug/lane). PDK2 (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin fixed and paraffin embedded skeletal muscle reacted with PDK2 Antibody Cat.-No AP53243PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells using PDK2 Antibody Cat.-No AP53243PU-N (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.