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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NB100-57851 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-57851, RRID:AB_2090815
- Product name
- Goat Polyclonal DGAT2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is not expected to cross-react with other DGAT members.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Reduced delta-6 desaturase activity partially protects against high-fat diet-induced impairment in whole-body glucose tolerance.
Novel effects of rosiglitazone on SMAD2 and SMAD3 signaling in white adipose tissue of diabetic rats.
Cloning of DGAT2, a second mammalian diacylglycerol acyltransferase, and related family members.
Hucik B, Sarr O, Nakamura MT, Dyck DJ, Mutch DM
The Journal of nutritional biochemistry 2019 May;67:173-181
The Journal of nutritional biochemistry 2019 May;67:173-181
Novel effects of rosiglitazone on SMAD2 and SMAD3 signaling in white adipose tissue of diabetic rats.
Beaudoin MS, Snook LA, Arkell AM, Stefanson A, Wan Z, Simpson JA, Holloway GP, Wright DC
Obesity (Silver Spring, Md.) 2014 Jul;22(7):1632-42
Obesity (Silver Spring, Md.) 2014 Jul;22(7):1632-42
Cloning of DGAT2, a second mammalian diacylglycerol acyltransferase, and related family members.
Cases S, Stone SJ, Zhou P, Yen E, Tow B, Lardizabal KD, Voelker T, Farese RV Jr
The Journal of biological chemistry 2001 Oct 19;276(42):38870-6
The Journal of biological chemistry 2001 Oct 19;276(42):38870-6
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DGAT2 Antibody [NB100-57851] - Analysis of DGAT2 in Human Liver lysate in the third lane (35ug protein in RIPA buffer) using NB100-57851 at 0.5ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: DGAT2 Antibody [NB100-57851] - With increasing amount of recombinant DGAT2 captured by a rabbit antibody. Recommended reporter concentration: 0.5 - 1 ug/mL.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DGAT2 Antibody [NB100-57851] - Analysis of DGAT2 in Human Liver. Antibody at 3 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: DGAT2 Antibody [NB100-57851] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
