GTX632189

antibody from GeneTex

Targeting: ME1

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX632189

Provider

GeneTex

Product name

ME1 antibody [GT979]

Antibody type

Monoclonal

Reactivity

Human, Mouse

Host

Mouse

Western blot

Enhanced validation

Submitted by

GeneTex (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ME1 antibody [GT979] (GTX632189) diluted at 1:1000.

KD Reagent Type

siRNA

Downregulation

>50%

KD Reagent Prod page

KD Reagent Prod page

KD Reagent Provider

shRNA reagents was provided courtesy of cooperative labs.

KD Reagent Prod no

Proprietary.

Antibody Lot Number

42037

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Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ME1 antibody detects ME1 protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ME1 antibody (GTX632189) diluted at a dilution of 1:1000.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ME1 antibody [GT979] (GTX632189) diluted at 1:1000.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ME1 antibody [GT979] detects ME1 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ME1 protein stained by ME1 antibody [GT979] (GTX632189) diluted at 1:400.Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ME1 antibody [GT979] detects ME1 protein at cytoplasm in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. ME1 antibody [GT979] (GTX632189) diluted at 1:250.