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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00004199-M03 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00004199-M03, RRID:AB_2143826
- Product name
- Mouse Monoclonal ME1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. ME1 - malic enzyme 1, NADP(+)-dependent, cytosolic
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Role for malic enzyme, pyruvate carboxylation, and mitochondrial malate import in glucose-stimulated insulin secretion.
Heart E, Cline GW, Collis LP, Pongratz RL, Gray JP, Smith PJ
American journal of physiology. Endocrinology and metabolism 2009 Jun;296(6):E1354-62
American journal of physiology. Endocrinology and metabolism 2009 Jun;296(6):E1354-62
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ME1 Antibody (3H5) [H00004199-M03] - Analysis of ME1 expression in transfected 293T cell line by ME1 monoclonal antibody (M03), clone 3H5. Lane 1: ME1 transfected lysatE (64.1 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ME1 Antibody (3H5) [H00004199-M03] - Analysis of ME1 expression in HepG2 (Cat # L019V1).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: ME1 Antibody (3H5) [H00004199-M03] - Detection limit for recombinant GST tagged ME1 is approximately 1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ME1 Antibody (3H5) [H00004199-M03] - Analysis of monoclonal antibody to ME1 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 0.5 ug/ml
