Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Other assay [1]
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Validation data
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- Product number
- 18285-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DDX19B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MGFNRPSKI QENALPLMLA EPPQNLIAQS QSGTGKTAAF VLAMLSQVEP ANKYPQCLCL SPTYELALQT GKVIEQMGKF YPELKLAYAV RGNKLERGQK ISEQIVIGTP GTVLDWCSKL KFIDPKKIKV FVLDEADVMI ATQGHQDQSI RIQRMLPRNC QMLLFSATFE DSVWKFAQKV VPDPNVIKLK REEETLDTIK QYYVLCSSRD EKFQALCNLY GAITIAQAMI FCHTRKTASW LAAELSKEGH QVALLSGEMM VEQRAAVIER FREGKEKVLV TTNVCARGID VEQVSVVINF DLPVDKDGNP DNETYLHRIG RTGRFGKRGL AVNMVDSKHS MNILNRIQEH FNKKIERLDT DDLDEIEKIA N (1-370 aa encoded by BC010008)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.19 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 18285-1-AP (DDX19B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18285-1-AP (DDX19B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 18285-1-AP (DDX19B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 18285-1-AP (DDX19B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-DDX19B (IP:18285-1-AP, 4ug; Detection:18285-1-AP 1:500) with K-562 cells lysate 4000ug.