- NB100-760 - Novus Biologicals DDX19B antibody

Submitted references DDX19 Inhibits Type I Interferon Production by Disrupting TBK1-IKKε-IRF3 Interactions and Promoting TBK1 and IKKε Degradation.

Zhang K, Zhang Y, Xue J, Meng Q, Liu H, Bi C, Li C, Hu L, Yu H, Xiong T, Yang Y, Cui S, Bu Z, He X, Li J, Huang L, Weng C
Cell reports 2019 Jan 29;26(5):1258-1272.e4

Influenza A Virus Polymerase Recruits the RNA Helicase DDX19 to Promote the Nuclear Export of Viral mRNAs.

Diot C, Fournier G, Dos Santos M, Magnus J, Komarova A, van der Werf S, Munier S, Naffakh N
Scientific reports 2016 Sep 22;6:33763

RNA export factor Ddx19 is required for nuclear import of the SRF coactivator MKL1.

Rajakylä EK, Viita T, Kyheröinen S, Huet G, Treisman R, Vartiainen MK
Nature communications 2015 Jan 14;6:5978

Nuclear export factor RBM15 facilitates the access of DBP5 to mRNA.

Zolotukhin AS, Uranishi H, Lindtner S, Bear J, Pavlakis GN, Felber BK
Nucleic acids research 2009 Nov;37(21):7151-62

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: DDX19B Antibody [NB100-760] - Detection of Human and Mouse. Whole cell lysate from HeLa (5, 15 and 50 ug), 293T (T; 50 ug) and NIH3T3 (M; 50 ug) cells. Antibody used at 0.04 ug/ml.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: DDX19B Antibody [NB100-760] - Ddx19 binds directly to MKL1 RPEL domain. Recruitment of Ipobeta and Ddx19 from Ipobeta-depleted NIH 3T3 cytoplasmic lysates by GST-RPEL. (-), NIH 3T3 cytoplasmic lysate transfected with control siRNAs; (+), Ipobeta-depleted NIH 3T3 cytoplasmic lysate. Ponceau-stained full membrane (below). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/ncomms6978), licensed under a CC-BY licence.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: DDX19B Antibody [NB100-760] - MKL1 contains two Ddx19 interaction sites. HA-tagged MKL1 constructs were immunoprecipitated from NIH 3T3 lysates and their expression was detected by HA-antibody. Co-precipitated endogenous Ddx19 was detected by western blotting (WB). Empty HA was used as a negative control. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/ncomms6978), licensed under a CC-BY licence.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: DDX19B Antibody [NB100-760] - Ddx19 is specifically required for the nuclear localization of MKL1. Western blotting (WB) of cells treated with control (Ctrl) or Ddx19 siRNAs (si). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/ncomms6978), licensed under a CC-BY licence.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: DDX19B Antibody [NB100-760] - Ddx19 interacts with MKL1 RPEL domain and Ipobeta. Ddx19 recruitment from Hela cell lysate by GST-RPEL and GST-Ipobeta (left). WB, western blotting; (-), without Ran-Q69L; (+), with RanQ69L. Corresponding GST baits are stained with Ponceau to ensure the equal loading of the samples (below). Input sample corresponds to 5% of the Hela cell lysate used in the assay. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/ncomms6978), licensed under a CC-BY licence.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: DDX19B Antibody [NB100-760] - Ddx19 interacts with MKL1 RPEL domain and Ipobeta. GST-RPEL was used as a bait for pull down of Ddx19 and Ipobeta from the Hela cell lysate in the presence of increasing amounts of LatB-actin (0.25-10 uM) (left). Arrow indicates the increasing amounts of actin in the corresponding Ponceau staining (below). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/ncomms6978), licensed under a CC-BY licence.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry: DDX19B Antibody [NB100-760] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DDX19 used at a dilution of 1:200 ( 1ug/ml). Detection: DAB

NB100-760

Antibody data