24944-1-AP

antibody from Invitrogen Antibodies

Targeting: ME2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

24944-1-AP

Provider

Invitrogen Antibodies

Product name

ME2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: SISDRGHVR SIVDNWPENH VKAVVVTDGE RILGLGDLGV YGMGIPVGKL CLYTACAGIR PDRCLPVCID VGTDNIALLK DPFYMGLYQK RDRTQQYDDL IDEFMKAITD RYGRNTLIQF EDFGNHNAFR FLRKYREKYC TFNDDIQGTA AVALAGLLAA QKVISKPISE HKILFLGAGE AALGIANLIV MSMVENGLSE QEAQKKIWMF DKYGLLVKGR KAKIDSYQEP FTHSAPESIP DTFEDAVNIL KPSTIIGVAG AGRLFTPDVI RAMASINERP VIFALSNPTA QAECTAEEAY TLTEGRCLFA SGSPFGPVKL TDGRVFTPGQ GNNVYIFPGY RIPIC (136-480 aa encoded by BC000147)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.4 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 24944-1-AP (ME2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HL-60 cells were subjected to SDS PAGE followed by western blot with 24944-1-AP (ME2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 24944-1-AP ( ME2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 24944-1-AP (ME2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 24944-1-AP (ME2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon tissue slide using 24944-1-AP (ME2 Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon tissue slide using 24944-1-AP (ME2 Antibody) at dilution of 1:200 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 24944-1-AP ( ME2 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 24944-1-AP ( ME2 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-ME2 (IP:24944-1-AP, 4ug; Detection:24944-1-AP 1:500) with HeLa cells lysate 3200ug.