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antibody from Invitrogen Antibodies
Targeting: BTAF1
MOT1, TAF-172, TAF(II)170, TAF172, TAFII170
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Chromatin Immunoprecipitation [1]
- Other assay [1]
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- Product number
- PA5-96434 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TAF172 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LAVQNSSLHD IQHAPKLSAL KQLLLDCGLG NGSTSESGTE SVVAQHRILI FCQLKSMLDI VEHDLLKPHL PSVTYLRLDG SIPPGQRHSI VSRFNNDPSI DVLLLTTHVG GLGLNLTGAD TVVFVEHDWN PMRDLQAMDR AHRIGQKRVV NVYRLITRGT LEEKIMGLQK FKMNIANTVI SQENSSLQSM GTDQLLDLFT LDKDGKAEKA DTSTSGKASM KSILENLSDL WDQEQYDSEY SLENFMHSLK; Positive Samples: HepG2, HeLa, MCF7, Rat testis; Cellular Location: Nucleus
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.57 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TAF172 in extracts of various cell lines using TAF172 Polyclonal Antibody (Product # PA5-96434) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Chromatin immunoprecipitation analysis of TAF172 in HeLa using TAF172 Polyclonal Antibody (Product # PA5-96434) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of TAF172 was performed in 150 µg extracts of HeLa cells using TAF172 Polyclonal Antibody (Product # PA5-96434). Western blot was performed from the immunoprecipitate using TAF172 Polyclonal Antibody at a dilution of 1:500.
