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LearnA301-064A
antibody from Invitrogen Antibodies
Targeting: BTAF1
MOT1, TAF-172, TAF(II)170, TAF172, TAFII170
Western blot
Immunoprecipitation
Other assayAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Other assay [1]
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- Product number
- A301-064A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BTAF1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Detection of human BTAF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BTAF1 antibody A301-064A used for IP at 3 µg/mg lysate (A and B). BTAF1 was also immunoprecipitated by rabbit anti-BTAF1 antibodies A301-063A and A301-065A, which recognize other epitopes. For blotting immunoprecipitated BTAF1, A301-063A (A) and A301-066A (B) were used at 1 µg/ml,. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 30 seconds (B).
