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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- H00054961-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00054961-M01, RRID:AB_2271009
- Product name
- Mouse Monoclonal SSH3 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. SSH3 - slingshot homolog 3 (Drosophila)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Systematic identification of novel SLE related autoantibodies responsible for type I IFN production in human plasmacytoid dendritic cells.
Kassim SH, Jordan J, Schreiter J, Adhikarakunnathu S, Baribaud F, San Mateo L
Cellular immunology 2013 Jul-Aug;284(1-2):119-28
Cellular immunology 2013 Jul-Aug;284(1-2):119-28
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SSH3 Antibody (6F9) [H00054961-M01] - Analysis of SSH3 expression in transfected 293T cell line by SSH3 monoclonal antibody (M01), clone 6F9.Lane 1: SSH3 transfected lysate(73 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SSH3 Antibody (6F9) [H00054961-M01] - SSH3 monoclonal antibody (M01), clone 6F9 Analysis of SSH3 expression in A-431.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: SSH3 Antibody (6F9) [H00054961-M01] - Detection limit for recombinant GST tagged SSH3 is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: SSH3 Antibody (6F9) [H00054961-M01] - Analysis of monoclonal antibody to SSH3 on A-431 cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: SSH3 Antibody (6F9) [H00054961-M01] - Analysis of SSH3 transfected lysate using anti-SSH3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SSH3 monoclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SSH3 Antibody (6F9) [H00054961-M01] - Analysis of monoclonal antibody to SSH3 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 1 ~ 10 ug/ml.
