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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- NB100-60675 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-60675, RRID:AB_922514
- Product name
- Rabbit Polyclonal SSH3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Knockdown of Slingshot 2 (SSH2) serine phosphatase induces Caspase3 activation in human carcinoma cell lines with the loss of the Birt-Hogg-Dubé tumour suppressor gene (FLCN).
Lu X, Boora U, Seabra L, Rabai EM, Fenton J, Reiman A, Nagy Z, Maher ER
Oncogene 2014 Feb 20;33(8):956-65
Oncogene 2014 Feb 20;33(8):956-65
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SSH3 Antibody [NB100-60675] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. NB100-60675 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. SSH3 was also immunoprecipitated by rabbit anti-SSH3 antibody NB100-60674, which recognizes an upstream epitope.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: SSH3 Antibody [NB100-60675] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- SSH3 used at a dilution of 1:200 (1ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SSH3 Antibody [NB100-60675] - FFPE section of human colon carcinoma. Affinity purified rabbit anti-SSH3 used at a dilution of 1:250.
