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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP54050PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP54050PU-N, RRID:AB_11146001
- Product name
- anti SSH3 (C-term)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 582-610 amino acids from the C-terminal region of Human SSH3
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of SSH3 Antibody (C-term) Cat.-No AP54050PU-N in Hela cell line lysates (35ug/lane).This demonstrates the SSH3 antibody detected the SSH3 protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of SSH3 Antibody (C-term) Cat.-No AP54050PU-N with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin fixed, paraffin embedded human breast carcinoma stained with SSH3 Antibody (C-term) Cat.-No AP54050PU-N, wihc was peroxidase conjugated to the secondary antibody and followed by DAB staining.This data demonstrates the use of SSH3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Hela cells using SSH3 Antibody (C-term) Cat.-No AP54050PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
