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antibody from Invitrogen Antibodies
Targeting: FADS2
D6D, DES6, FADSD6, LLCDL2, SLL0262, TU13
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- PA5-48353 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FADS2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Predicted to react with monkey based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Expression of SCD and FADS2 Is Lower in the Necrotic Core and Growing Tumor Area than in the Peritumoral Area of Glioblastoma Multiforme.
Korbecki J, Kojder K, Jeżewski D, Simińska D, Tarnowski M, Kopytko P, Safranow K, Gutowska I, Goschorska M, Kolasa-Wołosiuk A, Wiszniewska B, Chlubek D, Baranowska-Bosiacka I
Biomolecules 2020 May 7;10(5)
Biomolecules 2020 May 7;10(5)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FADS2 in in HepG2 cell line lysates (35µg/lane). Samples were probed with a FADS2 Antibody (N-term) (Product # PA5-48353).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorecent analysis of FADS2 in A549 cells. Cells were stained with FADS2 polyclonal Antibody (N-term) (Product # PA5-48353) at a 1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/mL, 1 h at 37°C).FADS2 immunoreactivity is localized to Cytoplasm and Vesicles significantly.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of FADS2 in formalin fixed and paraffin embedded human liver tissue. Samples stained using a FADS2 Antibody (N-term) (Product # PA5-48353), followed by peroxidase conjugation of the secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of FADS2 in K562 cells (right histogram) compared to a negative control cell (left histogram). Samples were probed with a FADS2 Antibody (N-term) (Product # PA5-48353). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Representative microphotographs showing the protein expression of desaturase FADS2 in neurons and glia cells in the peritumoral area ( A ; red and white arrows) and in cells in the growing tumor area ( B ) necrotic foci (NF) ( C , D ; blue arrows) of brain tumors from patients diagnosed with glioblastoma. Please note that on microphotography of GTA/NC, there are many pathologically changed regions of the brain tissue that indicate brain cell necrosis envelope by hypercellular zones (HcZ), with additional microvascular hyperplasia (green arrows). There are microphotographs from different objective (obj. magnification: A , x20, C x20, B , D x40); scale bars are 200 um or 100 um.
