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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-20140 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-20140, RRID:AB_2101095
- Product name
- Anti-FASN
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
Submitted references C/EBPalpha and C/EBPbeta are required for Sebocyte differentiation and stratified squamous differentiation in adult mouse skin.
Dysregulation of sterol regulatory element binding protein-1c in livers of morbidly obese women is associated with altered suppressor of cytokine signaling-3 and signal transducer and activator of transcription-1 signaling.
ULK-Atg13-FIP200 complexes mediate mTOR signaling to the autophagy machinery.
Down-regulation of transcription factor peroxisome proliferator-activated receptor in programmed hepatic lipid dysregulation and inflammation in intrauterine growth-restricted offspring.
House JS, Zhu S, Ranjan R, Linder K, Smart RC
PloS one 2010 Mar 23;5(3):e9837
PloS one 2010 Mar 23;5(3):e9837
Dysregulation of sterol regulatory element binding protein-1c in livers of morbidly obese women is associated with altered suppressor of cytokine signaling-3 and signal transducer and activator of transcription-1 signaling.
Elam MB, Yellaturu C, Howell GE, Deng X, Cowan GS, Kumar P, Park EA, Hiler ML, Wilcox HG, Hughes TA, Cook GA, Raghow R
Metabolism: clinical and experimental 2010 Apr;59(4):587-98
Metabolism: clinical and experimental 2010 Apr;59(4):587-98
ULK-Atg13-FIP200 complexes mediate mTOR signaling to the autophagy machinery.
Jung CH, Jun CB, Ro SH, Kim YM, Otto NM, Cao J, Kundu M, Kim DH
Molecular biology of the cell 2009 Apr;20(7):1992-2003
Molecular biology of the cell 2009 Apr;20(7):1992-2003
Down-regulation of transcription factor peroxisome proliferator-activated receptor in programmed hepatic lipid dysregulation and inflammation in intrauterine growth-restricted offspring.
Magee TR, Han G, Cherian B, Khorram O, Ross MG, Desai M
American journal of obstetrics and gynecology 2008 Sep;199(3):271.e1-5
American journal of obstetrics and gynecology 2008 Sep;199(3):271.e1-5
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 220, 112, 84, 47, 32, 26, 16.8
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 273
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human cerebellum shows distinct cytoplasmic positivity in Purkinje cells.
- Validation comment
- Staining pattern consistent with experimental and/or bioinformatic data.
