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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [23]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NB400-114 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB400-114, RRID:AB_10002181
- Product name
- Rabbit Polyclonal Fatty Acid Synthase/FASN Antibody
- Antibody type
- Polyclonal
- Description
- Unpurified.
- Reactivity
- Human, Mouse, Rat, Chicken/Avian, Feline, Hamster, Porcine, Simian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Aspirin Improves Nonalcoholic Fatty Liver Disease and Atherosclerosis through Regulation of the PPARδ-AMPK-PGC-1α Pathway in Dyslipidemic Conditions.
Impact of discontinuation of fish oil after pioglitazone-fish oil combination therapy in diabetic KK mice.
Speckle-type POZ protein suppresses lipid accumulation and prostate cancer growth by stabilizing fatty acid synthase.
Adipocyte OGT governs diet-induced hyperphagia and obesity.
Fatty acid synthase as a potential therapeutic target in feline oral squamous cell carcinoma.
The Korean herbal medicine, Do In Seung Gi-Tang, attenuates atherosclerosis via AMPK in high-fat diet-induced ApoE(-/-) mice.
Andrographolide inhibits adipogenesis of 3T3-L1 cells by suppressing C/EBPβ expression and activation.
A human genome-wide loss-of-function screen identifies effective chikungunya antiviral drugs.
P300 acetyltransferase regulates fatty acid synthase expression, lipid metabolism and prostate cancer growth.
Histological and immunohistochemical markers for progression prediction in transurethrally resected high-grade non-muscle invasive bladder cancer.
Ursolic acid inhibits adipogenesis in 3T3-L1 adipocytes through LKB1/AMPK pathway.
Marine- and plant-derived ω-3 fatty acids differentially regulate prostate cancer cell proliferation.
Liver kinase b1 is required for white adipose tissue growth and differentiation.
Modulatory role of PYY in transport and metabolism of cholesterol in intestinal epithelial cells.
Proteasome inhibitor treatment reduced fatty acid, triacylglycerol and cholesterol synthesis.
A selective estrogen receptor α agonist ameliorates hepatic steatosis in the male aromatase knockout mouse.
LXRalpha activation perturbs hepatic insulin signaling and stimulates production of apolipoprotein B-containing lipoproteins.
Metabolic effects of dietary cholesterol in an animal model of insulin resistance and hepatic steatosis.
Mechanisms of antidiabetogenic and body weight-lowering effects of estrogen in high-fat diet-fed mice.
1-11C-acetate as a PET radiopharmaceutical for imaging fatty acid synthase expression in prostate cancer.
Androgen deprivation increases p300 expression in prostate cancer cells.
Long-term administration of estradiol decreases expression of hepatic lipogenic genes and improves insulin sensitivity in ob/ob mice: a possible mechanism is through direct regulation of signal transducer and activator of transcription 3.
FAS inhibitor cerulenin reduces food intake and melanocortin receptor gene expression without modulating the other (an)orexigenic neuropeptides in chickens.
Han YM, Lee YJ, Jang YN, Kim HM, Seo HS, Jung TW, Jeong JH
BioMed research international 2020;2020:7806860
BioMed research international 2020;2020:7806860
Impact of discontinuation of fish oil after pioglitazone-fish oil combination therapy in diabetic KK mice.
Iizuka Y, Chiba K, Kim H, Hirako S, Wada M, Matsumoto A
The Journal of nutritional biochemistry 2020 Feb;76:108265
The Journal of nutritional biochemistry 2020 Feb;76:108265
Speckle-type POZ protein suppresses lipid accumulation and prostate cancer growth by stabilizing fatty acid synthase.
Gang X, Xuan L, Zhao X, Lv Y, Li F, Wang Y, Wang G
The Prostate 2019 Jun;79(8):864-871
The Prostate 2019 Jun;79(8):864-871
Adipocyte OGT governs diet-induced hyperphagia and obesity.
Li MD, Vera NB, Yang Y, Zhang B, Ni W, Ziso-Qejvanaj E, Ding S, Zhang K, Yin R, Wang S, Zhou X, Fang EX, Xu T, Erion DM, Yang X
Nature communications 2018 Nov 30;9(1):5103
Nature communications 2018 Nov 30;9(1):5103
Fatty acid synthase as a potential therapeutic target in feline oral squamous cell carcinoma.
Walz JZ, Saha J, Arora A, Khammanivong A, O'Sullivan MG, Dickerson EB
Veterinary and comparative oncology 2018 Mar;16(1):E99-E108
Veterinary and comparative oncology 2018 Mar;16(1):E99-E108
The Korean herbal medicine, Do In Seung Gi-Tang, attenuates atherosclerosis via AMPK in high-fat diet-induced ApoE(-/-) mice.
Park SH, Sung YY, Jang S, Nho KJ, Choi GY, Kim HK
BMC complementary and alternative medicine 2016 Sep 8;16:352
BMC complementary and alternative medicine 2016 Sep 8;16:352
Andrographolide inhibits adipogenesis of 3T3-L1 cells by suppressing C/EBPβ expression and activation.
Chen CC, Chuang WT, Lin AH, Tsai CW, Huang CS, Chen YT, Chen HW, Lii CK
Toxicology and applied pharmacology 2016 Sep 15;307:115-122
Toxicology and applied pharmacology 2016 Sep 15;307:115-122
A human genome-wide loss-of-function screen identifies effective chikungunya antiviral drugs.
Karlas A, Berre S, Couderc T, Varjak M, Braun P, Meyer M, Gangneux N, Karo-Astover L, Weege F, Raftery M, Schönrich G, Klemm U, Wurzlbauer A, Bracher F, Merits A, Meyer TF, Lecuit M
Nature communications 2016 May 12;7:11320
Nature communications 2016 May 12;7:11320
P300 acetyltransferase regulates fatty acid synthase expression, lipid metabolism and prostate cancer growth.
Gang X, Yang Y, Zhong J, Jiang K, Pan Y, Karnes RJ, Zhang J, Xu W, Wang G, Huang H
Oncotarget 2016 Mar 22;7(12):15135-49
Oncotarget 2016 Mar 22;7(12):15135-49
Histological and immunohistochemical markers for progression prediction in transurethrally resected high-grade non-muscle invasive bladder cancer.
Kim K, Cho YM, Park BH, Lee JL, Ro JY, Go H, Shim JW
International journal of clinical and experimental pathology 2015;8(1):743-50
International journal of clinical and experimental pathology 2015;8(1):743-50
Ursolic acid inhibits adipogenesis in 3T3-L1 adipocytes through LKB1/AMPK pathway.
He Y, Li Y, Zhao T, Wang Y, Sun C
PloS one 2013;8(7):e70135
PloS one 2013;8(7):e70135
Marine- and plant-derived ω-3 fatty acids differentially regulate prostate cancer cell proliferation.
Eser PO, Vanden Heuvel JP, Araujo J, Thompson JT
Molecular and clinical oncology 2013 May;1(3):444-452
Molecular and clinical oncology 2013 May;1(3):444-452
Liver kinase b1 is required for white adipose tissue growth and differentiation.
Zhang W, Wang Q, Song P, Zou MH
Diabetes 2013 Jul;62(7):2347-58
Diabetes 2013 Jul;62(7):2347-58
Modulatory role of PYY in transport and metabolism of cholesterol in intestinal epithelial cells.
Grenier E, Garofalo C, Delvin E, Levy E
PloS one 2012;7(7):e40992
PloS one 2012;7(7):e40992
Proteasome inhibitor treatment reduced fatty acid, triacylglycerol and cholesterol synthesis.
Oliva J, French SW, Li J, Bardag-Gorce F
Experimental and molecular pathology 2012 Aug;93(1):26-34
Experimental and molecular pathology 2012 Aug;93(1):26-34
A selective estrogen receptor α agonist ameliorates hepatic steatosis in the male aromatase knockout mouse.
Chow JD, Jones ME, Prelle K, Simpson ER, Boon WC
The Journal of endocrinology 2011 Sep;210(3):323-34
The Journal of endocrinology 2011 Sep;210(3):323-34
LXRalpha activation perturbs hepatic insulin signaling and stimulates production of apolipoprotein B-containing lipoproteins.
Basciano H, Miller A, Baker C, Naples M, Adeli K
American journal of physiology. Gastrointestinal and liver physiology 2009 Aug;297(2):G323-32
American journal of physiology. Gastrointestinal and liver physiology 2009 Aug;297(2):G323-32
Metabolic effects of dietary cholesterol in an animal model of insulin resistance and hepatic steatosis.
Basciano H, Miller AE, Naples M, Baker C, Kohen R, Xu E, Su Q, Allister EM, Wheeler MB, Adeli K
American journal of physiology. Endocrinology and metabolism 2009 Aug;297(2):E462-73
American journal of physiology. Endocrinology and metabolism 2009 Aug;297(2):E462-73
Mechanisms of antidiabetogenic and body weight-lowering effects of estrogen in high-fat diet-fed mice.
Bryzgalova G, Lundholm L, Portwood N, Gustafsson JA, Khan A, Efendic S, Dahlman-Wright K
American journal of physiology. Endocrinology and metabolism 2008 Oct;295(4):E904-12
American journal of physiology. Endocrinology and metabolism 2008 Oct;295(4):E904-12
1-11C-acetate as a PET radiopharmaceutical for imaging fatty acid synthase expression in prostate cancer.
Vāvere AL, Kridel SJ, Wheeler FB, Lewis JS
Journal of nuclear medicine : official publication, Society of Nuclear Medicine 2008 Feb;49(2):327-34
Journal of nuclear medicine : official publication, Society of Nuclear Medicine 2008 Feb;49(2):327-34
Androgen deprivation increases p300 expression in prostate cancer cells.
Heemers HV, Sebo TJ, Debes JD, Regan KM, Raclaw KA, Murphy LM, Hobisch A, Culig Z, Tindall DJ
Cancer research 2007 Apr 1;67(7):3422-30
Cancer research 2007 Apr 1;67(7):3422-30
Long-term administration of estradiol decreases expression of hepatic lipogenic genes and improves insulin sensitivity in ob/ob mice: a possible mechanism is through direct regulation of signal transducer and activator of transcription 3.
Gao H, Bryzgalova G, Hedman E, Khan A, Efendic S, Gustafsson JA, Dahlman-Wright K
Molecular endocrinology (Baltimore, Md.) 2006 Jun;20(6):1287-99
Molecular endocrinology (Baltimore, Md.) 2006 Jun;20(6):1287-99
FAS inhibitor cerulenin reduces food intake and melanocortin receptor gene expression without modulating the other (an)orexigenic neuropeptides in chickens.
Dridi S, Ververken C, Hillgartner FB, Arckens L, Van der Gucht E, Cnops L, Decuypere E, Buyse J
American journal of physiology. Regulatory, integrative and comparative physiology 2006 Jul;291(1):R138-47
American journal of physiology. Regulatory, integrative and comparative physiology 2006 Jul;291(1):R138-47
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Fatty Acid Synthase/FASN Antibody [NB400-114] - Expression of Fatty Acid Synthase in chicken hypothalamus. A: total RNA (1 ug) isolated from different tissues (brain, hypothalamus, liver and muscle) was subjected to RT-PCR using specific primers for chicken Fatty Acid Synthase (GenBank accession JO4485) or ribosomal 18S as a control (GenBank accession AF173612) B: tissue lysates (brain, hypothalmus, liver and muscle) were subjected to Western blot as described in MATERIALS AND METHODS. Blots were incubated with anti-Fatty Acid Synthase antibody and revealed by enhanced chemiluminescence. Picture compliments of Dridi S. et al, Am J Physiol Regul Integr Comp Physiol. 2006 Jul;291(1):R138-47. Epub 2006 Feb 2.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Fatty Acid Synthase/FASN Antibody [NB400-114] - Analysis of Fatty Acid Synthase, using NB400-114. Samples: 50 ug of total mouse liver lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody [NB400-114] - Immunohistochemical localization of FAS in chicken hypothalmus. Paraffin sections were obtained from 3-wk-old broiler chickens.
