Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00002194-M02 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00002194-M02, RRID:AB_489957
- Product name
- FASN monoclonal antibody (M02), clone 3B3-1D6
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant FASN.
- Antigen sequence
MSTNDTIVSGTLPQRMASCLEVLDLFLNQPHMVLS
SFVLAEKAAAYRDRDSQRDLVEAVAHILGIRDLAA
VNLDSSLADLGLDSLMSVEVRQTLERELNLVLSVR
EVRQLTLRKLQELSSKADEASELACPTPKEDGLAQ
QQTQLNLRSLLVNPEGPTLMRLNSVQSSERPLFLV
HPIEGSTTVFHSLASGLSIPTYGLQCTRAAPLDSI
HSLAAYYIDCIRQVQPEGPYRVAGYSYGACVAFEM
CSQLQAQQSPAPTHNSLFLFDGSPTYVLAYTQSYR
AKLTPGCEAEAETEAICFFVQQFTDMEHNRVLEAL
LPLKGLEERVAAAVDLIIKSHQGLDRQELSFAARS
FYYKLRAAEQYTPKAKYHGNVMLLRAKTGGAYGED
LGADYNLSQVCDGKVSVHVIEGDHRTLLEGSGLES
IISIIHSSLAEPRVSVREG- Isotype
- IgG
- Antibody clone number
- 3B3-1D6
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references AMPK-sensed cellular energy state regulates the release of extracellular Fatty Acid Synthase.
Oliveras-Ferraros C, Vazquez-Martin A, Fernández-Real JM, Menendez JA
Biochemical and biophysical research communications 2009 Jan 16;378(3):488-93
Biochemical and biophysical research communications 2009 Jan 16;378(3):488-93
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of FASN expression in transfected 293T cell line by FASN monoclonal antibody (M02), clone 3B3-1D6.Lane 1: FASN transfected lysate(48.3 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- FASN monoclonal antibody (M02), clone 3B3-1D6. Western Blot analysis of FASN expression in human pancreas.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to FASN on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
