687302

antibody from BioLegend

Targeting: ITK EMT, LYK, PSCTK2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

687302

Provider

BioLegend

Proper citation

BioLegend Cat#687302, RRID:AB_2616942

Product name

Purified anti-ITK

Antibody type

Monoclonal

Antigen

Partial human ITK recombinant protein (1-200 a.a.) expressed in E. coli.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

W15110A

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Total cell lysate from human Raji cells (lane 1), HeLa cells (lane 2), and Jurkat cells (lane 3) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-ITK (clone W15110A) antibody. Proteins were visualized using an HRP Goat anti-mouse IgG antibody and chemiluminescence detection. Purified anti-GAPDH (clone Poly6314) antibody was used as a loading control.

Conjugate

Unconjugated