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LearnR34882
antibody from NSJ Bioreagents
Targeting: DAP3
bMRP-10, DAP-3, DKFZp686G12159, MGC126058, MGC126059, MRP-S29, MRPS29
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- R34882 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- DAP3 Antibody
- Antibody type
- Polyclonal
- Antigen
- Amino acids NPSLLERHCAYL were used as the immunogen for this DAP3 antibody. This sequence is common to isoforms 1, 2 and 3.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store the DAP3 antibody at -20°C.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot of HEK293 lysate overexpressing DAP3 probed with DAP3 antibody (mock transfection in lane 1). Predicted molecular weight: ~46/41/42kDa (isoforms 1/2/3).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot of HeLa lysate (control in left lane, post si-RNA-mediated DAP3 knock-down expresson in right lane) with DAP3 antibody at 1ug/ml. Level of knock-down relative to Actin expression level was determined by RT-PCR. Predicted molecular weight: ~46/41/42kDa (isoforms 1/2/3).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC staining of guanidinium thiocyanate-treated HeLa cells before (left) and after (right) si-RNA-mediated DAP3 knock-down using DAP3 antibody at 3ug/ml. Detection by DyLight 488 (green); nuclear DAPI stain (blue).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human MCF7 cells with DAP3 antibody at 5ug/ml.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human brain cortex tissue with DAP3 antibody at 2.5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human tonsil tissue with DAP3 antibody at 2.5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
