10685-1-AP

antibody from Invitrogen Antibodies

Targeting: MBIP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

10685-1-AP

Provider

Invitrogen Antibodies

Product name

MBIP Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MAAATEHNR PSSGDRNLER RCSPNLSREV LYEIFRSLHT LVGQLDLRDD VVKITIDWNK LQSLSAFQPA LLFSALEQHI LYLQPFLAKL QSPIKEENTT AVEEIGRTEM GNKNEVNDKF SIGDLQEEEK HKESDLRDVK KTQIHFDPEV VQIKAGKAEI DRRISAFIER KQAEINENNV REFCNVIDCN QENSCARTDA IFTPYPGFKS HVKVSRVVYT YGPQTRPEGI PGSGHKPNSM LRDCGNQAVE ERLQNIEAHL RLQTGGPVPR DIYQRIKKLE DKILELEGIS PEYFQSVSFS GKRRKVQPPQ QNYSLAELDE KISALKQALL RKSREAESMA THHLP (1-344 aa encoded by BC005197)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.14 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of MBIP antibody (10685-1-AP; 1:600; room temperature for 1.5 hours) with sh-Control and sh-MBIP transfected HEK-293 cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 10685-1-AP (MBIP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 10685-1-AP (MBIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 10685-1-AP (MBIP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 10685-1-AP (MBIP Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 10685-1-AP (MBIP Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis tissue slide using 10685-1-AP ( MBIP Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis tissue slide using 10685-1-AP ( MBIP Antibody) at dilution of 1:50 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-MBIP (IP:10685-1-AP, 4ug; Detection:10685-1-AP 1:300) with HEK-293 cells lysate 2800ug.