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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
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Validation data
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- Product number
- 500-P154G-1MG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-12 p70 Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: p35 subunit: RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNAS p40 subunit: IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS .
- Concentration
- 0.1-1.0 mg/mL
Submitted references Broadly active zinc finger protein-guided transcriptional activation of HIV-1.
The Global Phosphorylation Landscape of SARS-CoV-2 Infection.
CRACR2A-Mediated TCR Signaling Promotes Local Effector Th1 and Th17 Responses.
Systems analysis of latent HIV reversal reveals altered stress kinase signaling and increased cell death in infected T cells.
Modeling HIV-1 Latency in Primary T Cells Using a Replication-Competent Virus.
Multidirectional interactions are bridging human NK cells with plasmacytoid and monocyte-derived dendritic cells during innate immune responses.
CCR4 ligands are up-regulated in the airways of atopic asthmatics after segmental allergen challenge.
Potential mechanisms of human natural killer cell expansion in vivo during low-dose IL-2 therapy.
Scott TA, O'Meally D, Grepo NA, Soemardy C, Lazar DC, Zheng Y, Weinberg MS, Planelles V, Morris KV
Molecular therapy. Methods & clinical development 2021 Mar 12;20:18-29
Molecular therapy. Methods & clinical development 2021 Mar 12;20:18-29
The Global Phosphorylation Landscape of SARS-CoV-2 Infection.
Bouhaddou M, Memon D, Meyer B, White KM, Rezelj VV, Correa Marrero M, Polacco BJ, Melnyk JE, Ulferts S, Kaake RM, Batra J, Richards AL, Stevenson E, Gordon DE, Rojc A, Obernier K, Fabius JM, Soucheray M, Miorin L, Moreno E, Koh C, Tran QD, Hardy A, Robinot R, Vallet T, Nilsson-Payant BE, Hernandez-Armenta C, Dunham A, Weigang S, Knerr J, Modak M, Quintero D, Zhou Y, Dugourd A, Valdeolivas A, Patil T, Li Q, Hüttenhain R, Cakir M, Muralidharan M, Kim M, Jang G, Tutuncuoglu B, Hiatt J, Guo JZ, Xu J, Bouhaddou S, Mathy CJP, Gaulton A, Manners EJ, Félix E, Shi Y, Goff M, Lim JK, McBride T, O'Neal MC, Cai Y, Chang JCJ, Broadhurst DJ, Klippsten S, De Wit E, Leach AR, Kortemme T, Shoichet B, Ott M, Saez-Rodriguez J, tenOever BR, Mullins RD, Fischer ER, Kochs G, Grosse R, García-Sastre A, Vignuzzi M, Johnson JR, Shokat KM, Swaney DL, Beltrao P, Krogan NJ
Cell 2020 Aug 6;182(3):685-712.e19
Cell 2020 Aug 6;182(3):685-712.e19
CRACR2A-Mediated TCR Signaling Promotes Local Effector Th1 and Th17 Responses.
Woo JS, Srikanth S, Kim KD, Elsaesser H, Lu J, Pellegrini M, Brooks DG, Sun Z, Gwack Y
Journal of immunology (Baltimore, Md. : 1950) 2018 Aug 15;201(4):1174-1185
Journal of immunology (Baltimore, Md. : 1950) 2018 Aug 15;201(4):1174-1185
Systems analysis of latent HIV reversal reveals altered stress kinase signaling and increased cell death in infected T cells.
Fong LE, Sulistijo ES, Miller-Jensen K
Scientific reports 2017 Nov 23;7(1):16179
Scientific reports 2017 Nov 23;7(1):16179
Modeling HIV-1 Latency in Primary T Cells Using a Replication-Competent Virus.
Martins LJ, Bonczkowski P, Spivak AM, De Spiegelaere W, Novis CL, DePaula-Silva AB, Malatinkova E, Trypsteen W, Bosque A, Vanderkerckhove L, Planelles V
AIDS research and human retroviruses 2016 Feb;32(2):187-93
AIDS research and human retroviruses 2016 Feb;32(2):187-93
Multidirectional interactions are bridging human NK cells with plasmacytoid and monocyte-derived dendritic cells during innate immune responses.
Della Chiesa M, Romagnani C, Thiel A, Moretta L, Moretta A
Blood 2006 Dec 1;108(12):3851-8
Blood 2006 Dec 1;108(12):3851-8
CCR4 ligands are up-regulated in the airways of atopic asthmatics after segmental allergen challenge.
Pilette C, Francis JN, Till SJ, Durham SR
The European respiratory journal 2004 Jun;23(6):876-84
The European respiratory journal 2004 Jun;23(6):876-84
Potential mechanisms of human natural killer cell expansion in vivo during low-dose IL-2 therapy.
Fehniger TA, Bluman EM, Porter MM, Mrózek E, Cooper MA, VanDeusen JB, Frankel SR, Stock W, Caligiuri MA
The Journal of clinical investigation 2000 Jul;106(1):117-24
The Journal of clinical investigation 2000 Jul;106(1):117-24
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human IL-12 by Western Blot analysis IL-12 p70 Polyclonal Antibody (Product # 500-P154G-1MG) can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-12 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human IL-12 by Western Blot analysis IL-12 p70 Polyclonal Antibody (Product # 500-P154G-1MG) can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-12 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA: To detect Human IL-12 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of IL-12 p70 Polyclonal Antibody (Product # 500-P154G-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech IL-12 p70 Polyclonal Antibody, Biotin (Product # 500-P154GBT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-12.
