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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- MA5-14482 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD3e Monoclonal Antibody (EP449E)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- MA5-14482 targets CD3E in IHC (P) and Western blot applications and shows reactivity with Human and Mouse samples.
- Antibody clone number
- EP449E
- Concentration
- Conc. Not Determined
Submitted references Immune cell constitution in the tumor microenvironment predicts the outcome in diffuse large B-cell lymphoma.
PD-L1 Expression in Endometrial Carcinoma Cells and Intratumoral Immune Cells: Differences Across Histologic and TCGA-based Molecular Subgroups.
Alteration of hepatic structure and oxidative stress induced by intravenous nanoceria.
Hepatitis C virus induces the expression of CCL17 and CCL22 chemokines that attract regulatory T cells to the site of infection.
Autio M, Leivonen SK, Brück O, Mustjoki S, Mészáros Jørgensen J, Karjalainen-Lindsberg ML, Beiske K, Holte H, Pellinen T, Leppä S
Haematologica 2021 Mar 1;106(3):718-729
Haematologica 2021 Mar 1;106(3):718-729
PD-L1 Expression in Endometrial Carcinoma Cells and Intratumoral Immune Cells: Differences Across Histologic and TCGA-based Molecular Subgroups.
Pasanen A, Ahvenainen T, Pellinen T, Vahteristo P, Loukovaara M, Bützow R
The American journal of surgical pathology 2020 Feb;44(2):174-181
The American journal of surgical pathology 2020 Feb;44(2):174-181
Alteration of hepatic structure and oxidative stress induced by intravenous nanoceria.
Tseng MT, Lu X, Duan X, Hardas SS, Sultana R, Wu P, Unrine JM, Graham U, Butterfield DA, Grulke EA, Yokel RA
Toxicology and applied pharmacology 2012 Apr 15;260(2):173-82
Toxicology and applied pharmacology 2012 Apr 15;260(2):173-82
Hepatitis C virus induces the expression of CCL17 and CCL22 chemokines that attract regulatory T cells to the site of infection.
Riezu-Boj JI, Larrea E, Aldabe R, Guembe L, Casares N, Galeano E, Echeverria I, Sarobe P, Herrero I, Sangro B, Prieto J, Lasarte JJ
Journal of hepatology 2011 Mar;54(3):422-31
Journal of hepatology 2011 Mar;54(3):422-31
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD3E was performed by loading 25 µg of Jurkat (Lane 1), CEM (Lane 2) and mouse spleen (Lane 3) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a CD3E monoclonal antibody (Product # MA5-14482) at a dilution of 1:20 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at approx. 20 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human tonsil stained with CD3 Epsilon using peroxidase-conjugate and DAB chromogen. Note membrane staining.
