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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- ALX-804-834-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-804-834-C100, RRID:AB_2051177
- Product name
- CTRP2 (mouse) monoclonal antibody (Didoo-1)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- Didoo-1
- Vial size
- 100 μg
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20¡C.
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Supportive validation
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- Enzo Life Sciences (provider)
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- Experimental details
- Detection of mouse CTRP2 with MAb to CTRP2 (Didoo-1) (Prod. No. ALX-804-834) by Western blot. Method: Cell extracts (5x10E5) from HEK 293T( lane1) or HEK293wt transfected with a plasmid coding for mouse CTRP2 (lane 2) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the MAb to CTRP2 (Didoo-1) (1:1'000) for 2 hours, followed by incubation with HRP-coupled anti-mouse secondary antibody (1:4'000).
