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LearnAF782
antibody from R&D Systems
Targeting: IL22
IL-21, IL-22, IL-D110, IL-TIF, ILTIF, MGC79382, MGC79384, TIFa, TIFIL-23, zcyto18
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF782 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-22 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human, recombinant mouse, and recombinant rat IL-22 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human IL-10 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9GZX6- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Regulatory mechanisms of collagen expression by interleukin-22 signaling in scleroderma fibroblasts.
Interleukin-22 and Cyclosporine in Aggressive Cutaneous Squamous Cell Carcinoma.
Interleukin-22 is increased in multiple sclerosis patients and targets astrocytes.
Patients with cystic fibrosis have inducible IL-17+IL-22+ memory cells in lung draining lymph nodes.
Persistent Helicobacter pylori specific Th17 responses in patients with past H. pylori infection are associated with elevated gastric mucosal IL-1β.
IL-22 increases the innate immunity of tissues.
Sawamura S, Jinnin M, Inoue K, Yamane K, Honda N, Kajihara I, Makino T, Masuguchi S, Fukushima S, Ihn H
Journal of dermatological science 2018 Apr;90(1):52-59
Journal of dermatological science 2018 Apr;90(1):52-59
Interleukin-22 and Cyclosporine in Aggressive Cutaneous Squamous Cell Carcinoma.
Santana AL, Felsen D, Carucci JA
Dermatologic clinics 2017 Jan;35(1):73-84
Dermatologic clinics 2017 Jan;35(1):73-84
Interleukin-22 is increased in multiple sclerosis patients and targets astrocytes.
Perriard G, Mathias A, Enz L, Canales M, Schluep M, Gentner M, Schaeren-Wiemers N, Du Pasquier RA
Journal of neuroinflammation 2015 Jun 16;12:119
Journal of neuroinflammation 2015 Jun 16;12:119
Patients with cystic fibrosis have inducible IL-17+IL-22+ memory cells in lung draining lymph nodes.
Chan YR, Chen K, Duncan SR, Lathrop KL, Latoche JD, Logar AJ, Pociask DA, Wahlberg BJ, Ray P, Ray A, Pilewski JM, Kolls JK
The Journal of allergy and clinical immunology 2013 Apr;131(4):1117-29, 1129.e1-5
The Journal of allergy and clinical immunology 2013 Apr;131(4):1117-29, 1129.e1-5
Persistent Helicobacter pylori specific Th17 responses in patients with past H. pylori infection are associated with elevated gastric mucosal IL-1β.
Serelli-Lee V, Ling KL, Ho C, Yeong LH, Lim GK, Ho B, Wong SB
PloS one 2012;7(6):e39199
PloS one 2012;7(6):e39199
IL-22 increases the innate immunity of tissues.
Wolk K, Kunz S, Witte E, Friedrich M, Asadullah K, Sabat R
Immunity 2004 Aug;21(2):241-54
Immunity 2004 Aug;21(2):241-54
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human IL-22 by Western Blot. Western blot shows lysates of human tonsil tissue and human breast cancer tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human IL-22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF782) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IL-22 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-22 in Human PBMCs. IL-22 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with lipopolysaccharide (LPS) using 10 µg/mL Goat Anti-Human IL-22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF782) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-10 Secretion Induced by IL-22 and Neutralization by Human IL-22 Antibody. Recombinant Human IL-22 (Catalog # 782-IL) stimulates IL-10 secretion in the COLO 205 human colorectal adeno-carcinoma cell line in a dose-dependent manner (orange line), as measured by the Human IL-10 DuoSet ELISA Development Kit (Catalog # DY217B). IL-10 secretion elicited by Recombinant Human IL-22 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF782). The ND50 is typically 0.5-2.5 µg/mL.
