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LearnMAB617-100
antibody from R&D Systems
Targeting: CD40LG
CD154, CD40L, gp39, hCD40L, HIGM1, IMD3, TNFSF5, TRAP
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Immunohistochemistry [1]
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- Product number
- MAB617-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CD40 Ligand/TNFSF5 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human CD40 Ligand/TNFSF5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) CD27 Ligand, rhCD30 Ligand, or recombinant mouse CD40 Ligand is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P29965- Isotype
- IgG
- Antibody clone number
- 40804
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Design, Synthesis, and Evaluation of Novel Immunomodulatory Small Molecules Targeting the CD40⁻CD154 Costimulatory Protein-Protein Interaction.
Up-Regulated Expression of Matrix Metalloproteinases in Endothelial Cells Mediates Platelet Microvesicle-Induced Angiogenesis.
Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
Bojadzic D, Chen J, Alcazar O, Buchwald P
Molecules (Basel, Switzerland) 2018 May 11;23(5)
Molecules (Basel, Switzerland) 2018 May 11;23(5)
Up-Regulated Expression of Matrix Metalloproteinases in Endothelial Cells Mediates Platelet Microvesicle-Induced Angiogenesis.
Sun C, Feng SB, Cao ZW, Bei JJ, Chen Q, Zhao WB, Xu XJ, Zhou Z, Yu ZP, Hu HY
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;41(6):2319-2332
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;41(6):2319-2332
Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S
European journal of immunology 2008 Apr;38(4):1012-23
European journal of immunology 2008 Apr;38(4):1012-23
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CD40 Ligand/TNFSF5 in Human Tonsil. CD40 Ligand/TNFSF5 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD40 Ligand/TNFSF5 Monoclonal Antibody (Catalog # MAB617) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
