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Learn16668-1-AP
antibody from Invitrogen Antibodies
Targeting: CD40LG
CD154, CD40L, gp39, hCD40L, HIGM1, IMD3, TNFSF5, TRAP
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- 16668-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD40L/CD154 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MIETYNQTS PRSAATGLPI SMKIFMYLLT VFLITQMIGS ALFAVYLHRR LDKIEDERNL HEDFVFMKTI QRCNTGERSL SLLNCEEIKS QFEGFVKDIM LNKEETKKEN SFEMQKGDQN PQIAAHVISE ASSKTTSVLQ WAEKGYYTMS NNLVTLENGK QLTVKRQGLY YIYAQVTFCS NREASSQAPF IASLCLKSPG RFERILLRAA NTHSSAKPCG QQSIHLGGVF ELQPGASVFV NVTDPSQVSH GTGFTSFGLL KL (1-261 aa encoded by BC071754)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.3 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 16668-1-AP (CD40LG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 16668-1-AP (CD40LG antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 16668-1-AP (CD40L; CD154 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 16668-1-AP (CD154 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human spleen tissue slide using 16668-1-AP ( CD154 antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human spleen tissue slide using 16668-1-AP ( CD154 antibody) at dilution of 1:200 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-CD154 (IP:16668-1-AP, 4ug; Detection:16668-1-AP 1:1000) with Jurkat cells lysate 3200ug.
