310812

antibody from BioLegend

Targeting: CD40LG CD154, CD40L, gp39, hCD40L, HIGM1, IMD3, TNFSF5, TRAP

Immunocytochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Recommended by provider)

Antibody Data

Product number

310812

Provider

BioLegend

Proper citation

BioLegend Cat#310812, RRID:AB_314835

Product name

LEAF™ anti-human CD154 [24-31]

Antibody type

Monoclonal

Antigen

CD154

Description

CD154 (CD40 ligand) is also known as CD40L, gp39, TRAP and T-BAM. CD40 ligand is a 32-39 kD type II transmembrane glycoprotein. It is a member of the TNF superfamily and is expressed on activated T cells. It has been reported to be important for B cell costimulation following binding of its receptor, CD40. Additionally, binding of CD40L to CD40 on B cells promotes the secretion of immunoglobulin and Ig isotype switching. CD40L is also involved in the regulation of cytokine production by T cells.

Reactivity

Human, Simian

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

500 µg

Concentration

1 mg/ml

Storage

The CD154 antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.

References

Submitted references

Polypyrimidine tract-binding protein is critical for the turnover and subcellular distribution of CD40 ligand mRNA in CD4+ T cells.

Matus-Nicodemos R, Vavassori S, Castro-Faix M, Valentin-Acevedo A, Singh K, Marcelli V, Covey LR
Journal of immunology (Baltimore, Md. : 1950) 2011 Feb 15;186(4):2164-71

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Natural killer cells trigger differentiation of monocytes into dendritic cells.

Zhang AL, Colmenero P, Purath U, Teixeira de Matos C, Hueber W, Klareskog L, Tarner IH, Engleman EG, Söderström K
Blood 2007 Oct 1;110(7):2484-93

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

PMA+ionomycin-stimulated human PBMCs (6 hours) stained with 24-31 PE

Conjugate

Unconjugated