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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- MAB8396 - Provider product page
- Provider
- R&D Systems
- Product name
- Human MRGPRF Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects HEK293 human embryonic kidney cell line transfected with human MRGPRF by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NP_659452- Isotype
- IgG
- Antibody clone number
- 916219
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human MRGPRF by Western Blot. Western blot shows lysates of human uterus tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MRGPRF Monoclonal Antibody (Catalog # MAB8396) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MRGPRF at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of MRGPRF in HEK293 Human Cell Line Transfected with Human MRGPRF and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human MRGPRF or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human MRGPRF Monoclonal Antibody (Catalog # MAB8396) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002).
