SM5143P

antibody from Acris Antibodies GmbH

Targeting: CASR FHH, GPRC2A, HHC, HHC1, NSHPT

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

SM5143P

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#SM5143P, RRID:AB_973558

Product name

anti CaSR

Antibody type

Monoclonal

Antigen

Synthetic peptide corresponding to amino acids 214-235 of Human Calcium Sensing Receptor (CaSR).

Reactivity

Human, Mouse, Rat, Bovine

Host

Mouse

Isotype

IgG

Antibody clone number

5C10, ADD

Vial size

0.1 mg

Concentration

1.0 mg/ml

References

Submitted references

Novel inactivating mutations of the calcium-sensing receptor: the calcimimetic NPS R-568 improves signal transduction of mutant receptors.

Rus R, Haag C, Bumke-Vogt C, Bähr V, Mayr B, Möhlig M, Schulze E, Frank-Raue K, Raue F, Schöfl C
The Journal of clinical endocrinology and metabolism 2008 Dec;93(12):4797-803

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot of calcium sensing receptor in HEK7-2 cell extract using SM5143P.

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunofluorescence of Bovine CE (Corneal Epithelium) Limbus tissue extracts using SM5143P.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemical analysis of mouse stomach stained with Calcium Sensing Receptor Monoclonal Antibody (Cat.-No SM5143P). Fresh stomach tissue was fixed in 4% formalin for 1 hour and then incubated overnight at 4ºC in 25% sucrose before embedding in tissue freezing medium. Antigen retrieval was carried out on 8µm cryo-sections by incubating in sodium citrate buffer for 45 minutes at 4ºC, immersing in sodium citrate buffer for 10 minutes at 100ºC before washing 3 times for 5 minutes each in 1X PBS. Sections were then blocked in blocking buffer (0.3% Triton X-100 in 1X PBS containing 10% normal goat serum) for 30 minutes at RT before staining with SM5143P (diluted 1:100 in blocking buffer) overnight at 4ºC followed by a fluorophore-conjugated goat anti-mouse IgG secondary antibody for 2 hours at RT. Sections were also stained with DAPI nuclear stain (blue). SM5143P positive cells (green) were found at the base of the antral glands in the mouse stomach. Data courtesy of the Innovators Program.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry performed on normal biopsies of deparaffinized Human kidney tissue using CaSR Antibody Cat.-No SM5143P (Clone 5C10, ADD)