AP14453C

antibody from Abgent

Targeting: SAMD4B FLJ10211, hSmaug2, MGC99832, SMGB

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP14453C

Provider

Abgent

Proper citation

Abgent Cat#AP14453c, RRID:AB_11137013

Product name

Mouse Pak4 Antibody (Center)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Mouse Pak4 Antibody (Center) (Cat. #AP14453c) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the Pak4 antibody detected the Pak4 protein (arrow).

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Mouse Pak4 Antibody (Center) (Cat. #AP14453c) western blot analysis in Neuro-2a cell line lysates (35ug/lane).This demonstrates the Pak4 antibody detected the Pak4 protein (arrow).

Primary Ab dilution

1:1000

Flow cytometry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Mouse Pak4 Antibody (Center) (Cat. #AP14453c) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50