- PA5-22326 - Invitrogen Antibodies GBA antibody

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of beta-glucosidase using 30 µg HepG2 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a beta-glucosidase polyclonal antibody (Product # PA5-22326) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western Blot analysis of GBA was performed by separating 30 µg of MCF-7 whole cell and membrane extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a GBA Polyclonal Antibody (Product # PA5-22326) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Knockdown of GBA was achieved by transfecting NIH:OVCAR-3 with GBA specific siRNAs (Silencer® select Product # S501316, S534768). Western blot analysis (Fig. a) was performed using Whole cell extracts from the GBA knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with GBA Polyclonal Antibody (Product # PA5-22326, 1:2000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to GBA.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot was performed using Anti-GBA Polyclonal Antibody (Product # PA5-22326) and a 60 kDa band corresponding to GBA was observed across the cell lines tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), Hep G2 (Lane 2), MCF7 (Lane 3), HEK-293 (Lane 4), U-87 MG (Lane 5), NIH:OVCAR-3 (Lane 6) and LNCaP (Lane 7) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).An uncharacterized band of ~40 kDa was also observed in Hep G2, HEK-293 and LNCaP.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: GBA Polyclonal Antibody detects GBA protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. GBA stained by GBA Polyclonal Antibody (Product # PA5-22326) diluted at 1:1,000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: GBA Polyclonal Antibody detects GBA protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. GBA stained by GBA Polyclonal Antibody (Product # PA5-22326) diluted at 1:1,000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemical analysis of paraffin-embedded human hepatoma, using GBA (Product # PA5-22326) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

PA5-22326