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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00006277-M10 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00006277-M10, RRID:AB_547931
- Product name
- Mouse Monoclonal S100A6 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. S100A6 - S100 calcium binding protein A6 (calcyclin)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Expression of S100 protein family members in normal skin and sweat gland tumors.
Aberrant expression of S100A6 and matrix metalloproteinase 9, but not S100A2, S100A4, and S100A7, is associated with epidermal carcinogenesis.
Identification of novel molecular markers through transcriptomic analysis in human fetal and adult corneal endothelial cells.
S100A expression in normal corneal-limbal epithelial cells and ocular surface squamous cell carcinoma tissue.
Zhu L, Okano S, Takahara M, Chiba T, Tu Y, Oda Y, Furue M
Journal of dermatological science 2013 Jun;70(3):211-9
Journal of dermatological science 2013 Jun;70(3):211-9
Aberrant expression of S100A6 and matrix metalloproteinase 9, but not S100A2, S100A4, and S100A7, is associated with epidermal carcinogenesis.
Zhu L, Kohda F, Nakahara T, Chiba T, Tsuji G, Hachisuka J, Ito T, Tu Y, Moroi Y, Uchi H, Furue M
Journal of dermatological science 2013 Dec;72(3):311-9
Journal of dermatological science 2013 Dec;72(3):311-9
Identification of novel molecular markers through transcriptomic analysis in human fetal and adult corneal endothelial cells.
Chen Y, Huang K, Nakatsu MN, Xue Z, Deng SX, Fan G
Human molecular genetics 2013 Apr 1;22(7):1271-9
Human molecular genetics 2013 Apr 1;22(7):1271-9
S100A expression in normal corneal-limbal epithelial cells and ocular surface squamous cell carcinoma tissue.
Li J, Riau AK, Setiawan M, Mehta JS, Ti SE, Tong L, Tan DT, Beuerman RW
Molecular vision 2011;17:2263-71
Molecular vision 2011;17:2263-71
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: S100A6 Antibody (6B5) [H00006277-M10] - Analysis of S100A6 expression in transfected 293T cell line by S100A6 monoclonal antibody (M10), clone 6B5. Lane 1: S100A6 transfected lysatE (10.2 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: S100A6 Antibody (6B5) [H00006277-M10] - Analysis of S100A6 expression in HeLa (Cat # L013V1).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: S100A6 Antibody (6B5) [H00006277-M10] - Detection limit for recombinant GST tagged S100A6 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: S100A6 Antibody (6B5) [H00006277-M10] - Analysis of monoclonal antibody to S100A6 on HeLa cell. Antibody concentration 10 ug/ml
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: S100A6 Antibody (6B5) [H00006277-M10] - Analysis of monoclonal antibody to S100A6 on formalin-fixed paraffin-embedded human stomach carcinoma. Antibody concentration 3 ug/ml
