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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [1]
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- Product number
- PAB11260 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB11260, RRID:AB_1714209
- Product name
- SLIT3 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of SLIT3.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Conserved modularity and potential for alternate splicing in mouse and human Slit genes.
Dual trafficking of Slit3 to mitochondria and cell surface demonstrates novel localization for Slit protein.
Identification of high-molecular-weight proteins with multiple EGF-like motifs by motif-trap screening.
Distinct but overlapping expression patterns of two vertebrate slit homologs implies functional roles in CNS development and organogenesis.
Little M, Rumballe B, Georgas K, Yamada T, Teasdale RD
The International journal of developmental biology 2002;46(4):385-91
The International journal of developmental biology 2002;46(4):385-91
Dual trafficking of Slit3 to mitochondria and cell surface demonstrates novel localization for Slit protein.
Little MH, Wilkinson L, Brown DL, Piper M, Yamada T, Stow JL
American journal of physiology. Cell physiology 2001 Aug;281(2):C486-95
American journal of physiology. Cell physiology 2001 Aug;281(2):C486-95
Identification of high-molecular-weight proteins with multiple EGF-like motifs by motif-trap screening.
Nakayama M, Nakajima D, Nagase T, Nomura N, Seki N, Ohara O
Genomics 1998 Jul 1;51(1):27-34
Genomics 1998 Jul 1;51(1):27-34
Distinct but overlapping expression patterns of two vertebrate slit homologs implies functional roles in CNS development and organogenesis.
Holmes GP, Negus K, Burridge L, Raman S, Algar E, Yamada T, Little MH
Mechanisms of development 1998 Dec;79(1-2):57-72
Mechanisms of development 1998 Dec;79(1-2):57-72
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot using SLIT3 polyclonal antibody (Cat # PAB11260) shows detection ofa predominant band at ~145 KDa corresponding to SLIT3 (arrowhead) in a bovine thyroid tissue lysate using the 800 nm channel (green).Approximately 35 ug of lysate was separated on a 4-8% Tricine gel by SDS-PAGE and transferred onto nitrocellulose.After blocking the membrane was probed with the primary antibody diluted to 1 : 800.Incubation was for 2 h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDye™ 800 conjugated Gt-a-RabbitIgG [H&L] MXHu for 45 min atroom temperature.Molecular weight markers were used for size comparison using the 700 nm channel (not shown).IRDye™ 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.IRDye isa trademark of LI-COR, Inc.
