MA1-91555

antibody from Invitrogen Antibodies

Targeting: S100A7 PSOR1, S100A7c

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-91555

Provider

Invitrogen Antibodies

Product name

S100A7 Monoclonal Antibody (47C1068)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

MA1-91555 has been successfully used in immunohistochemistry & Western blot applications, where a band of ~11 kDa is detected. Confluent MCF10A cells can be used as a positive control for this antibody.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

47C1068

Vial size

100 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Unique profile of antimicrobial peptide expression in polymorphic light eruption lesions compared to healthy skin, atopic dermatitis, and psoriasis.

Patra V, Mayer G, Gruber-Wackernagel A, Horn M, Lembo S, Wolf P
Photodermatology, photoimmunology & photomedicine 2018 Mar;34(2):137-144

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Psoriasin in A) MCF10A and B) MCF7 cell lysate using a Psoriasin monoclonal antibody (Product # MA1-91555) at a dilution of 1 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Psoriasin in confluent MCF10A cells using a Psoriasin monoclonal antibody (Product # MA1-91555) at a dilution of 1 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of S100A7 was performed by loading MCF-7 cell lysate. Proteins were transferred to a membrane and probed with a S100A7 Monoclonal Antibody (47C1068) (Product # MA1-91555).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of (A) MCF-10a and (B) MCF-7 cell lysate using S100A7 Monoclonal Antibody (47C1068) (Product # MA1-91555). Dilution: 1 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of S100A7 was performed by loading MCF-7 cell lysate. Proteins were transferred to a membrane and probed with a S100A7 Monoclonal Antibody (47C1068) (Product # MA1-91555).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of S100A7 in (A) untreated or (B) LPS-treated (100 ng/mL, 21 hrs) HOPE-fixed NCI-H727 cells using S100A7 Monoclonal Antibody (47C1068) (Product # MA1-91555). Positive control(C) is shown by the expression of the nuclear Ki67 antigen with the MIB-1 (2 µg/mL) antibody. Negative control(D) was included omitting the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of S100A7 in human breast tissue using S100A7 Monoclonal Antibody (47C1068) (Product # MA1-91555) at a dilution of 1:200. Antigen retrieval : Citrate buffer pH 6.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of S100A7 in human esophageal squamous cell carcinoma (SCC) tissue using S100A7 Monoclonal Antibody (47C1068) (Product # MA1-91555) at a dilution of 5 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of S100A7 in human lymph node cancer tissue using S100A7 Monoclonal Antibody (47C1068) (Product # MA1-91555) at a dilution of 5 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of S100A7 in human skin tissue using S100A7 Monoclonal Antibody (47C1068) (Product # MA1-91555) at a dilution of 10 µg/mL. Antigen retrieval : Heat-induced antigen retrieval.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of MCK10 was performed in Jurkat cells (fixed with 4% PFA, permeabilized with 0.1% saponin) using a MCK10 Polyclonal Antibody (Product # PA1-30315) (Blue) at a dilution of 1 µg/mL. Orange : isotype control.