PAB23786

antibody from Abnova Corporation

Targeting: KARS1 DFNB89, KARS, KARS2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB23786

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB23786, RRID:AB_11122608

Product name

KARS polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant KARS.

Antigen sequence

GMVKHITGSYKVTYHPDGPEGQAYDVDFTPPFRRI
NMVEELEKALGMKLPETNLFETEETRKILDDICVA
KAVECPPPRTTARLLDKLVGEFLEVTCINPTFICD
HPQIMSPLAKWHRSK


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with KARS polyclonal antibody (Cat # PAB23786) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with KARS polyclonal antibody (Cat # PAB23786) at 1-4 ug/mL dilution shows positivity in cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human hippocampus with KARS polyclonal antibody (Cat # PAB23786) shows strong cytoplasmic positivity in neuronal cells at 1:500-1:1000 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)