MA1-12626

antibody from Invitrogen Antibodies

Targeting: PGR NR3C3, PR

Antibody data

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Validation data

Product number: MA1-12626 - Provider product page
Provider: Invitrogen Antibodies
Product name: Progesterone Receptor Antibody Cocktail
Antibody type: Monoclonal
Antigen: Other
Description: MA1-12626 detects Progesterone Receptor in human, equine and porcine samples.
Antibody clone number: hPRa2, hPRa3
Concentration: 1 mg/mL

PGR protein structure - MA1-12626 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Progesterone Receptor (green) in T47D cells untreated (left) or stimulated with 100nm promegestone, R5020, for 1 hour (right). The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 3% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were stained with Progesterone Receptor mouse monoclonal antibody (Product # MA1-12626), at a dilution of 1:100 for 1 hour at 37C, and then incubated with a Alexa Fluor 488 Superclonal goat anti-mouse IgG secondary antibody (Product # A28175) at a dilution of 1:1000 for 30 minutes at room temperature (both panels, green). Nuclei (both panels, blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight at 20X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Progesterone Receptor was performed using 70% confluent log phase T-47D cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Progesterone Receptor Monoclonal Antibody (hPRa2 + hPRa3) (Product # MA1-12626) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. Panel f shows the absence of Progesterone Receptor expression in the negative model, SK-BR-3.The images were captured at 60X magnification.