209-301-E16

antibody from Rockland Immunochemicals, Inc.

Targeting: PGR NR3C3, PR

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

209-301-E16

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#209-301-E16, RRID:AB_11183136

Product name

Anti-Progesterone Receptor pS190 (Mouse) Monoclonal Antibody - 209-301-E16

Antibody type

Monoclonal

Vial size

100 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western Blot of Mouse anti-Progesterone Receptor pS190 antibody. Lane 1: whole cell T47D lysate prepared from cells that had been inubated in the presence of the synthetic progestin agonist R5020 (500 nM). Lane 2: none. Load: 10 µg per lane. Primary antibody: Progesterone Receptor pS190 antibody at 1:1,000 for overnight at 4°C. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 90 kDa for Progesterone Receptor pS190. Other band(s): Progesterone Receptor pS190 splice variants and isoforms.

Validation comment

Western Blot