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- Antibody Data
- Antigen structure
- References [4]
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- Immunohistochemistry [1]
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- Product number
- AF5110 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#AF5110, RRID:AB_341800
- Product name
- Sheep Polyclonal Reg3B Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse Reg3B in direct ELISAs and Western blots. In direct ELISAs, approximately 75% cross-reactivity with recombinant rat (rr) Reg3B is observed. In Western blots approximately 30% cross-reactivity with rrReg3B is observed. In direct ELISAs and Western blots, less than 1% cross-reactivity with rrReg3A, recombinant mouse (rm) Reg3A, and rmReg3G is observed.
- Reactivity
- Mouse
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Fetal exposure to maternal inflammation does not affect postnatal development of genetically-driven ileitis and colitis.
GM-CSF produced by nonhematopoietic cells is required for early epithelial cell proliferation and repair of injured colonic mucosa.
Mucin Muc2 deficiency and weaning influences the expression of the innate defense genes Reg3β, Reg3γ and angiogenin-4.
Urinary levels of regenerating islet-derived protein III β and gelsolin differentiate gentamicin from cisplatin-induced acute kidney injury in rats.
Hemmerling J, Heller K, Hörmannsperger G, Bazanella M, Clavel T, Kollias G, Haller D
PloS one 2014;9(5):e98237
PloS one 2014;9(5):e98237
GM-CSF produced by nonhematopoietic cells is required for early epithelial cell proliferation and repair of injured colonic mucosa.
Egea L, McAllister CS, Lakhdari O, Minev I, Shenouda S, Kagnoff MF
Journal of immunology (Baltimore, Md. : 1950) 2013 Feb 15;190(4):1702-13
Journal of immunology (Baltimore, Md. : 1950) 2013 Feb 15;190(4):1702-13
Mucin Muc2 deficiency and weaning influences the expression of the innate defense genes Reg3β, Reg3γ and angiogenin-4.
Burger-van Paassen N, Loonen LM, Witte-Bouma J, Korteland-van Male AM, de Bruijn AC, van der Sluis M, Lu P, Van Goudoever JB, Wells JM, Dekker J, Van Seuningen I, Renes IB
PloS one 2012;7(6):e38798
PloS one 2012;7(6):e38798
Urinary levels of regenerating islet-derived protein III β and gelsolin differentiate gentamicin from cisplatin-induced acute kidney injury in rats.
Ferreira L, Quiros Y, Sancho-Martínez SM, García-Sánchez O, Raposo C, López-Novoa JM, González-Buitrago JM, López-Hernández FJ
Kidney international 2011 Mar;79(5):518-28
Kidney international 2011 Mar;79(5):518-28
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Reg3B in Mouse Small Intestine. Reg3B was detected in immersion fixed frozen sections of mouse small intestine using 10 µg/mL Sheep Anti-Mouse Reg3B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5110) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
