MAB5110

antibody from R&D Systems

Targeting: REG3A HIP, PAP, PAP1, PBCGF, REG-III, REG3

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB5110

Provider

R&D Systems

Product name

Mouse Reg3B Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects mouse Reg3B in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant mouse (rm) Reg3A, rmReg3D, recombinant human Reg3D, recombinant rat (rr) Reg3B, or rrReg3G.

Reactivity

Mouse

Host

Rat

Conjugate

Unconjugated

Antigen sequence

P35230


Isotype

IgG

Antibody clone number

518630

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration.

Yui S, Azzolin L, Maimets M, Pedersen MT, Fordham RP, Hansen SL, Larsen HL, Guiu J, Alves MRP, Rundsten CF, Johansen JV, Li Y, Madsen CD, Nakamura T, Watanabe M, Nielsen OH, Schweiger PJ, Piccolo S, Jensen KB
Cell stem cell 2018 Jan 4;22(1):35-49.e7

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Lactobacillus salivarius reverse antibiotic-induced lung defense impairment in a ventilator model.

Tsay TB, Yang MC, Chang WH, Chen PH, Chen LW
Journal of translational medicine 2018 Aug 13;16(1):225

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Toll-like receptor stimulation induces nondefensin protein expression and reverses antibiotic-induced gut defense impairment.

Wu YY, Hsu CM, Chen PH, Fung CP, Chen LW
Infection and immunity 2014 May;82(5):1994-2005

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Diabetes aggravates acute pancreatitis and inhibits pancreas regeneration in mice.

Zechner D, Spitzner M, Bobrowski A, Knapp N, Kuhla A, Vollmar B
Diabetologia 2012 May;55(5):1526-34

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Mouse Reg3B by Western Blot. Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 2 µg/mL of Mouse Reg3B Monoclonal Antibody (Catalog # MAB5110) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Reg3B at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.