17635-1-AP

antibody from Invitrogen Antibodies

Targeting: RORB NR1F2, ROR-BETA, RZRB

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

17635-1-AP

Provider

Invitrogen Antibodies

Product name

RORB Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: EQRQQQSGE AEALARVYSS SISNGLSNLN NETSGTYANG HVIDLPKSEG YYNVDSGQPS PDQSGLDMTG IKQIKQEPIY DLTSVPNLFT YSSFNNGQLA PGITMTEIDR IAQNIIKSHL ETCQYTMEEL HQLAWQTHTY EEIKAYQSKS REALWQQCAI QITHAIQYVV EFAKRITGFM ELCQNDQILL LKSGCLEVVL VRMCRAFNPL NNTVLFEGKY GGMQMFKALG SDDLVNEAFD FAKNLCSLQL TEEEIALFSS AVLISPDRAW LIEPRKVQKL QEKIYFALQH VIQKNHLDDE TLAKLIAKIP TITAVCNLHG EKLQVFKQSH PEIVNTLFPP LYKELFNPDC ATGCK (106-459 aa encoded by BC051830)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.25 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Y79 cells were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Y79 cells were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Y79 cells were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 17635-1-AP (RORB antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 17635-1-AP (RORB Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 17635-1-AP (RORB Antibody) at dilution of 1:200 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 17635-1-AP (RORB antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-RORB (IP:17635-1-AP, 4ug; Detection:17635-1-AP 1:300) with mouse brain tissue lysate 3440ug.