Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [10]
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Validation data
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- Product number
- NBP1-83962 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-83962, RRID:AB_11032967
- Product name
- Rabbit Polyclonal ERGIC1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human ERGIC1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Stadler C, Rexhepaj E, Singan VR, Murphy RF, Pepperkok R, Uhlén M, Simpson JC, Lundberg E
Nature methods 2013 Apr;10(4):315-23
Nature methods 2013 Apr;10(4):315-23
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ERGIC1 Antibody [NBP1-83962] - Analysis in control (vector only transfected HEK293T lysate) and ERGIC1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ERGIC1 Antibody [NBP1-83962] - Staining of human prostate shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ERGIC1 Antibody [NBP1-83962] - Staining of human skin shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ERGIC1 Antibody [NBP1-83962] - Staining in human prostate and skin tissues using anti-ERGIC1 antibody. Corresponding ERGIC1 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ERGIC1 Antibody [NBP1-83962] - Staining of human testis.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ERGIC1 Antibody [NBP1-83962] - Staining of human liver.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ERGIC1 Antibody [NBP1-83962] - Staining of human cerebral cortex.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ERGIC1 Antibody [NBP1-83962] - Staining of human lymph node.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ERGIC1 Antibody [NBP1-83962] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ERGIC1 Antibody [NBP1-83962] - Staining of human cerebral cortex, liver, prostate and testis using Anti-ERGIC1 antibody NBP1-83962 (A) shows similar protein distribution across tissues to independent antibody NBP1-83963 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ERGIC1 Antibody [NBP1-83962] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.