AM33114PU-N

antibody from Acris Antibodies GmbH

Targeting: NAMPT PBEF, PBEF1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM33114PU-N

Provider

Acris Antibodies GmbH

Product name

anti Visfatin / NAMPT

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rat

Isotype

IgG

Antibody clone number

14A5

Vial size

0.1 mg

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

NAMPT is expressed in the cytoplasm. Equal amouns of cytosolic and membrane proteins from anti-CD3 stimulated spleen cells were separated by SDS-PAGE and blotted with 14A5 mAb (0. 1μg/ml). Secondary Ab: anti-Rat-HPRT:

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Cell extract from fetal mouse skin dendritic cell line (FSDC) was separated by SDS-PAGE and blotted with 14A5 mAb (0.1 μg/ml). Secondary Ab: anti Rat-HPRT

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

NAMPT is expressed in distinct spleen cell populations (CD11c positive and negative). Immunohistochemistry of mouse spleen frozen section (14A5: 10 μg /ml). Secondary Ab: anti-Rat-Alexa488, anti-CD11c-Alexa568

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunostaining of Mouse spleen frozen section (14A5: 10 μg /ml). Secondary Ab: Biotinylated anti-Rat