LS-C344720

antibody from LSBio

Targeting: NAMPT PBEF, PBEF1

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C344720

Provider

LSBio

Product name

NAMPT / Visfatin Antibody (clone OMNI379, Biotin) LS-C344720

Antibody type

Monoclonal

Description

Protein G purified from tissue culture supernatant

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Biotin

Isotype

IgG

Antibody clone number

OMNI379

Storage

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of 3T3-L1 cell lysate using anti-Nampt (Visfatin/PBEF) mAb (OMNI379) at 1: 2,000 dilution. 1. IBMX. + DEX. - treated 3T3-L1 cell lysate. 2. IBMX. + DEX. + Insulin - treated 3T3-L1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis using anti-Nampt (Visfatin-PBEF), mAb (OMNI379) at 1: 2,000 dilution. 1. Human Nampt(Visfatin/PBEF) (His-tagged). 2. Human Nampt(Visfatin/PBEF) (FLAG-tagged). 3. Mouse Nampt(Visfatin/PBEF) (His-tagged). 4. Rat Nampt(Visfatin/PBEF) (His-tagged). 5. Human Nampt(Visfatin/PBEF) (His-tagged). 6. LPS-treated human peripheral blood leukocyte lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitation of recombinant human Nampt(Visfatin/PBEF) proteins using anti-Nampt (Visfatin-PBEF), mAb (OMNI379). Recombinant human Nampt(Visfatin/PBEF) proteins at different concentrations were precipitated. The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by western blot with anti-human Nampt(Visfatin/PBEF) pAb. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemical staining of Nampt using anti-Nampt (OMNI379) (Biotin) in human tissue (1:500 dilution). Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney showing cytoplasmic staining (200x, brown color). This image was taken for the unconjugated form of this product. Other forms have not been tested.