A700-058

antibody from Invitrogen Antibodies

Targeting: NAMPT PBEF, PBEF1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A700-058

Provider

Invitrogen Antibodies

Product name

NAMPT/PBEF/Visfatin Recombinant Rabbit Monoclonal Antibody (BLR058F)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Antibody clone number

BLR058F

Vial size

100 µL

Concentration

250 µg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse NAMPT/PBEF/Visfatin by western blot. Samples: Whole cell lysate (50 µg) from RKO, MCF-7, K-562, GaMG, HEK293T, HeLa, U2OS, LNCaP, Hep-G2, Jurkat, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal antibody [BLR058F] (Product # A700-058 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-GAPDH (Product # A300-639A).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human NAMPT/PBEF/Visfatin by immunocytochemistry.Sample: FFPE section of NCI-H460.Antibody: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal antibody [BLR058F] (Product # A700-058 lot 1) used at 1:250.Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human NAMPT/PBEF/Visfatin by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma.Antibody: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal antibody [BLR058F] (Product # A700-058 lot 1) used at 1:250.Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human NAMPT/PBEF/Visfatin by immunohistochemistry. AntibodySample: FFPE section of human breast carcinoma.Antibody: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal antibody (BLR058F) (A700-058 lot 1) used at 1:250.Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human NAMPT/PBEF/Visfatin (shaded) in MOLT4 cells by flow cytometry. Antibody: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal [BLR058F] (Product #A700-058; lot 1) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG (Product # A120-201D5).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human NAMPT/PBEF/Visfatin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal antibody [BLR058F] (Product # A700-058 lot 1) used for IP at 5 µL per reaction. NAMPT/PBEF/Visfatin was also immunoprecipitated by rabbit anti-NAMPT/PBEF/Visfatin antibodies (Product # A300-779A and Product # A300-372A). For blotting immunoprecipitated NAMPT/PBEF/Visfatin (Product # A700-058) was used at 1:1000. Chemiluminescence with an exposure time of 75 seconds.