A700-058-T
antibody from Invitrogen Antibodies
Targeting: NAMPT
PBEF, PBEF1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- A700-058-T - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NAMPT/PBEF/Visfatin Recombinant Rabbit Monoclonal Antibody (BLR058F)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- BLR058F
- Vial size
- 10 µL
- Concentration
- 250 µg/mL
- Storage
- 4° C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse NAMPT/PBEF/Visfatin by western blot. Samples: Whole cell lysate (50 µg) from RKO, MCF-7, K-562, GaMG, HEK293T, HeLa, U2OS, LNCaP, Hep-G2, Jurkat, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal antibody [BLR058F] (Product # A700-058 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-GAPDH (Product # A300-639A).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human NAMPT/PBEF/Visfatin by immunocytochemistry.Sample: FFPE section of NCI-H460.Antibody: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal antibody [BLR058F] (Product # A700-058 lot 1) used at 1:250.Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human NAMPT/PBEF/Visfatin by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma.Antibody: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal antibody [BLR058F] (Product # A700-058 lot 1) used at 1:250.Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human NAMPT/PBEF/Visfatin by immunohistochemistry. AntibodySample: FFPE section of human breast carcinoma.Antibody: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal antibody (BLR058F) (A700-058 lot 1) used at 1:250.Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human NAMPT/PBEF/Visfatin (shaded) in MOLT4 cells by flow cytometry. Antibody: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal [BLR058F] (Product #A700-058; lot 1) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG (Product # A120-201D5).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human NAMPT/PBEF/Visfatin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal antibody [BLR058F] (Product # A700-058 lot 1) used for IP at 5 µL per reaction. NAMPT/PBEF/Visfatin was also immunoprecipitated by rabbit anti-NAMPT/PBEF/Visfatin antibodies (Product # A300-779A and Product # A300-372A). For blotting immunoprecipitated NAMPT/PBEF/Visfatin (Product # A700-058) was used at 1:1000. Chemiluminescence with an exposure time of 75 seconds.