GTX104644

antibody from GeneTex

Targeting: USP15 KIAA0529, UNPH4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX104644

Provider

GeneTex

Proper citation

GeneTex Cat#GTX104644, RRID:AB_1952527

Product name

USP15 antibody [C3], C-term

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ?g of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7.5% SDS PAGE GTX104644 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ?g of whole cell lysate) A: Molt-4 (GTX27912) 7.5% SDS PAGE GTX104644 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Whole cell extract (30 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with USP15 antibody [C3], C-term (GTX104644) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Whole cell extract (30 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with USP15 antibody [C3], C-term (GTX104644) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescence analysis of paraformaldehyde-fixed A431, using USP15(GTX104644) antibody at 1:200 dilution.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

USP15 antibody [C3], C-term detects USP15 protein by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: USP15 stained by USP15 antibody [C3], C-term (GTX104644) diluted at 1:500.Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded N87 xenograft, using USP15(GTX104644) antibody at 1:100 dilution.